The Effects of The Non-Fatal Electrical Injury on Caspase-3 Expression in Ovaries of Female Albino Rats: Histological and Immunohistochemical Study

Document Type : Original Article

Authors

1 Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Minia University, Minia, Egypt.

2 Department of Pathology, Faculty of Medicine, Minia University, Minia, Egypt.

Abstract

Introduction: In the developing world, electrical injury presents a serious issue with high morbidity
and mortality. Gonads are electrical current-sensitive organs because of their low resistance. Few
studies have been done on the gonads, even though many have shown the effects of electricity on
various body organs. Aim: This work aimed to detect the ovaries' histological changes and assess the
immunohistochemical expression of caspase-3 at different intervals (immediate, 2h, 24h, 72h & 7
days) in female albino rats that have been exposed to a non-lethal electrical current. Methodology:
Egypt, Minia, Faculty of Medicine, Department of Forensic Medicine and Clinical Toxicology is
where the current study was conducted. It contained 60 female albino rats, each weighing between
200 and 250 g. To analyze caspase-3 histopathologically and immunohistochemically, sections from
the ovaries were processed. Results: The studied animals' ovaries displayed the presence of ovarian
trauma with significant histological abnormalities and a significant increase in caspase-3
expression. Conclusion: Caspase-3 elevated levels and the histopathological changes supported the
theory that apoptosis was the main mechanism of the electrical injury to the ovaries. Future fertility
may be affected by these changes, which may manifest as abnormal ovarian function.
Recommendations: Further studies on the ovarian changes following electrical injury with different
time intervals than those used in the present research are recommended.

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